{"product_id":"kit-enzyme-temperature-optimum-study","title":"Enzyme Temperature Optimum Study Kit with Arduino Uno + LED","description":"\u003ch1\u003eEnzyme Temperature Optimum Study Kit with Arduino Uno \u0026amp; OLED — Find Reaction Sweet Spot\u003c\/h1\u003e\n\n\u003cp class=\"value-summary\"\u003eEvery part needed, pre-tested for compatibility, with an AI build companion trained on this exact project. Shipped from Bengaluru in 3-5 days.\u003c\/p\u003e\n\n\u003cdiv class=\"specs-strip\"\u003e\n  \u003cspan\u003e\u003cstrong\u003eDifficulty:\u003c\/strong\u003e Intermediate\u003c\/span\u003e\n  \u003cspan\u003e\u003cstrong\u003eBuild Time:\u003c\/strong\u003e 3-4 hrs\u003c\/span\u003e\n  \u003cspan\u003e\u003cstrong\u003eAge:\u003c\/strong\u003e 15-18\u003c\/span\u003e\n  \u003cspan\u003e\u003cstrong\u003eSkill:\u003c\/strong\u003e Enzyme kinetics data logging\u003c\/span\u003e\n\u003c\/div\u003e\n\n\u003cp\u003eEnzymes are biological catalysts whose activity depends heavily on temperature. This kit lets you precisely measure how an enzyme's reaction rate changes as you vary temperature, using two DS18B20 probes to monitor the enzyme solution and water bath, plus a pH module to track product formation. Whether for a CBSE class 12 investigatory project, a Smart India Hackathon prototype, or a B.Tech biotech lab, you'll gather real data and identify the enzyme's optimum temperature — just like in a professional research setup.\u003c\/p\u003e\n\n\u003ch2\u003eWhat You'll Build\u003c\/h2\u003e\n\u003cp\u003eBy the end of this 3–4 hour build, you'll have constructed a dual-zone temperature data logger with OLED display and microSD storage. The station uses one DS18B20 probe in the enzyme solution and a second in the water bath to maintain tight thermal control. The analog pH module continuously tracks product formation, so you can calculate reaction rate at every temperature point. All readings update in real time on the 0.96 inch OLED and save to a CSV file with millisecond timestamps from the DS3231 RTC.\u003c\/p\u003e\n\n\u003ch2\u003eWhat You'll Learn\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eCalibrating an analog pH module and converting its voltage output to pH units using buffer solutions\u003c\/li\u003e\n  \u003cli\u003eWiring and reading multiple DS18B20 temperature probes over OneWire with Arduino\u003c\/li\u003e\n  \u003cli\u003eStructuring time-stamped sensor data on a microSD card for analysis in Excel or Python\u003c\/li\u003e\n  \u003cli\u003ePlotting reaction rate vs. temperature curves to pinpoint the thermal optimum of an enzyme\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003ctable\u003e\n  \u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eQuantity\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n  \u003ctbody\u003e\n    \u003ctr\u003e\n\u003ctd\u003eArduino Uno R3\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003eDS18B20 Probe\u003c\/td\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003eAnalog pH Module\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003eDS3231 RTC\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003eMicroSD Module\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003e0.96in OLED\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003e4.7kΩ Resistors\u003c\/td\u003e\n\u003ctd\u003e5\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003e10kΩ Resistors\u003c\/td\u003e\n\u003ctd\u003e5\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003e400-pt Breadboard\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003eM-M Wires\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003e9V Battery Snap\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003ch2\u003eWhy Buy This Kit Instead of Sourcing Parts Separately\u003c\/h2\u003e\n\u003ctable\u003e\n  \u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eFactor\u003c\/th\u003e\n\u003cth\u003eSourcing Separately\u003c\/th\u003e\n\u003cth\u003eCompoden Kit\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n  \u003ctbody\u003e\n    \u003ctr\u003e\n\u003ctd\u003eCompatibility checks\u003c\/td\u003e\n\u003ctd\u003eYou verify every part\u003c\/td\u003e\n\u003ctd\u003ePre-tested as a system\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003eBuild support\u003c\/td\u003e\n\u003ctd\u003eForums and scattered tutorials\u003c\/td\u003e\n\u003ctd\u003eAI companion trained on this exact project\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003eTime to first working build\u003c\/td\u003e\n\u003ctd\u003eDays of debugging\u003c\/td\u003e\n\u003ctd\u003eHours, with step-by-step guidance\u003c\/td\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003eShipping coordination\u003c\/td\u003e\n\u003ctd\u003eMultiple sellers, multiple delays\u003c\/td\u003e\n\u003ctd\u003eOne shipment from Bengaluru in 3-5 days\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003ch2\u003eWho This Kit Is For\u003c\/h2\u003e\n\u003cp\u003eThis kit is designed for CBSE Class 11–12 students tackling biology or chemistry investigatory projects, B.Tech ECE\/Biotechnology undergrads at IITs, NITs, VIT, or BITS Pilani who need a plug-and-play data logging rig, and ATL Tinkering Lab mentors who want a science fusion project. It's equally relevant for Smart India Hackathon teams building precision monitoring prototypes. If you need to graph real enzyme kinetics data — not just simulate it — this kit delivers.\u003c\/p\u003e\n\n\u003ch2\u003eBuilt and Backed by Compoden\u003c\/h2\u003e\n\u003cp\u003eEvery Compoden kit ships with an AI build companion trained on this exact project — accessible via a QR code on the box, with WhatsApp and email backup. We've spent 10 years building projects for makers, schools, and institutions across India. If a part fails because of a manufacturing defect, replace it free within 7 days.\u003c\/p\u003e\n\n\u003cdetails\u003e\u003csummary\u003eWhat if I get stuck during the build?\u003c\/summary\u003e\u003cp\u003eOpen the AI companion by scanning the QR code on the box; it has troubleshooting steps for every component. You can also message us on WhatsApp — we reply within 2 hours during working days.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails\u003e\u003csummary\u003eHow do I control the temperature of the enzyme solution?\u003c\/summary\u003e\u003cp\u003eHeat water in a simple beaker on a stove or hot plate, then place the enzyme tube in the water bath. One DS18B20 goes inside the enzyme solution, the other in the water bath, so Arduino can compare and help you manually adjust the heat to hold each test temperature steady.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails\u003e\u003csummary\u003eCan I use any enzyme with this kit?\u003c\/summary\u003e\u003cp\u003eYes, but we provide a reference protocol for catalase (from liver or potato) and amylase (from saliva or germinating seeds), because these show clear pH changes during reaction. For other enzymes, ensure the pH module has a measurable output over your reaction's pH range.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails\u003e\u003csummary\u003eHow do I analyse the logged data?\u003c\/summary\u003e\u003cp\u003eThe microSD card stores a CSV file with columns for timestamp, pH, and both temperatures. Import this file into Google Sheets or Excel, calculate reaction rate (ΔpH\/time) for each temperature, and plot rate vs. temperature. The peak of the graph is the optimum temperature.\u003c\/p\u003e\u003c\/details\u003e\n\n\u003cdiv class=\"kit-description\"\u003e\n  \u003cp\u003eDS18B20 + pH track enzyme reaction at different temperatures. Finds optimum temperature.\u003c\/p\u003e\n  \u003ch4\u003eWhat's in this kit\u003c\/h4\u003e\n  \u003cul\u003e\n    \u003cli\u003e\u003ca href=\"\/products\/uno-r3-ch340g-atmega328p-board-arduino-compatible\"\u003eArduino Uno R3\u003c\/a\u003e\u003c\/li\u003e\n    \u003cli\u003e\n\u003ca href=\"\/products\/ds18b20-digital-temp-sensor-module-1-wire-55-to-125c\"\u003eDS18B20 Probe\u003c\/a\u003e x2\u003c\/li\u003e\n    \u003cli\u003e\u003ca href=\"\/products\/liquid-ph-0-14-sensor-module-with-bnc-probe-for-arduino\"\u003eAnalog pH Module\u003c\/a\u003e\u003c\/li\u003e\n    \u003cli\u003e\u003ca href=\"\/products\/ds3231-real-time-clock-module-i2c-rtc-with-battery-backup\"\u003eDS3231 RTC\u003c\/a\u003e\u003c\/li\u003e\n    \u003cli\u003e\u003ca href=\"\/products\/microsd-card-reader-spi-module-for-arduino\"\u003eMicroSD Module\u003c\/a\u003e\u003c\/li\u003e\n    \u003cli\u003e\u003ca href=\"\/products\/096in-oled-display-128x64-i2cspi-for-arduino-raspberry-pi\"\u003e0.96in OLED\u003c\/a\u003e\u003c\/li\u003e\n    \u003cli\u003e\n\u003ca href=\"\/products\/resistor-variety-pack-100-pcs-10-values-14w-carbon-film\"\u003e4.7kΩ Resistors\u003c\/a\u003e x5\u003c\/li\u003e\n    \u003cli\u003e\n\u003ca href=\"\/products\/resistor-variety-pack-100-pcs-10-values-14w-carbon-film\"\u003e10kΩ Resistors\u003c\/a\u003e x5\u003c\/li\u003e\n    \u003cli\u003e\u003ca href=\"\/products\/breadboard-standard-bundle-830400-tie-points-for-prototyping\"\u003e400-pt Breadboard\u003c\/a\u003e\u003c\/li\u003e\n    \u003cli\u003eM-M Wires x20\u003c\/li\u003e\n    \u003cli\u003e\u003ca href=\"\/products\/9v-battery-snap-connector-with-15cm-wires-compoden\"\u003e9V Battery Snap\u003c\/a\u003e\u003c\/li\u003e\n  \u003c\/ul\u003e\n\u003c\/div\u003e\n\n\u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"FAQPage\",\n  \"mainEntity\": [\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What is included in the Enzyme Temperature Optimum Study Kit with Arduino Uno + LED?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The Enzyme Temperature Optimum Study Kit with Arduino Uno + LED includes all components needed: Arduino Uno R3, DS18B20 Probe, Analog pH Module, DS3231 RTC, MicroSD Module and more. Everything is pre-tested for compatibility and shipped from Bengaluru, India.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What skill level is required for the Enzyme Temperature Optimum Study Kit with Arduino Uno + LED?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"This kit is designed for Intermediate level makers, suitable for ages 15-18. DS18B20 + pH track enzyme reaction at different temperatures. Finds optimum temperature. Estimated build time is 3-4 hrs.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Can I buy the Enzyme Temperature Optimum Study Kit with Arduino Uno + LED online in India?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Yes, the Enzyme Temperature Optimum Study Kit with Arduino Uno + LED is available online at Compoden (compoden.in), India's AI-powered electronics and robotics store. 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