Enzyme Kinetics Michaelis-Menten Kit with Arduino Uno + Sensor
Build a High-Precision Enzyme Kinetics Lab: Michaelis-Menten Km and Vmax Measurement Kit with Arduino Uno
Every part needed, pre-tested for compatibility, with an AI build companion trained on this exact project. Shipped from Bengaluru in 3-5 days.
This kit transforms an Arduino Uno into a scientific-grade enzyme assay platform, capable of measuring turbidity changes during an enzymatic reaction. With the included turbidity sensor and precise temperature control, you’ll collect data at multiple substrate concentrations, then mathematically derive the Michaelis-Menten constants Km and Vmax—just like in a professional biochemistry lab. By the end, you’ll hold a self-contained instrument that logs real-time product accumulation to an SD card and displays kinetics on an OLED screen.
What You'll Build
A fully functional spectrophotometric system that tracks turbidity over time, tracing product formation kinetics. You’ll design experiments with varying substrate levels, timestamp every reading with the DS3231 RTC, and export CSV datasets for non-linear regression. The assembled device maintains stable reaction conditions via the DS18B20 probe, ensuring reproducible results across multiple runs.
What You'll Learn
- Calibrating a turbidity sensor for biochemical assays using serial dilution standards
- Integrating a DS3231 real-time clock to log time-stamped sensor data to a microSD card
- Using a DS18B20 digital thermometer to monitor and maintain enzyme reaction temperature
- Applying Michaelis-Menten kinetics and non-linear curve fitting to experimental data to extract Km and Vmax
Kit Contents
| Component | Quantity |
|---|---|
| Arduino Uno R3 | 1 |
| Turbidity Sensor | 1 |
| DS18B20 Probe | 1 |
| DS3231 RTC | 1 |
| MicroSD Module | 1 |
| 0.96in OLED | 1 |
| 4.7kΩ Resistors | 5 |
| 100nF Caps | 5 |
| PCB Prototype Board | 2 |
| 9V Battery Snap | 1 |
| Soldering Iron | 1 |
| Solder Wire | 1 |
Why Buy This Kit Instead of Sourcing Parts Separately
| Factor | Sourcing Separately | Compoden Kit |
|---|---|---|
| Compatibility checks | You verify every part | Pre-tested as a system |
| Build support | Forums and scattered tutorials | AI companion trained on this exact project |
| Time to first working build | Days of debugging | Hours, with step-by-step guidance |
| Shipping coordination | Multiple sellers, multiple delays | One shipment from Bengaluru in 3-5 days |
Who This Kit Is For
Ideal for B.Tech ECE/EEE and M.Sc. Biotechnology/Biochemistry students building instrumentation projects, Smart India Hackathon teams developing point‑of‑care enzyme diagnostics, and ATL Tinkering Lab mentors guiding advanced STEM investigations. The kit also serves PhD researchers who need a low‑cost, logged experiment bed for enzyme kinetics method development before scaling to UV‑Vis spectrophotometers.
Built and Backed by Compoden
Every Compoden kit ships with an AI build companion trained on this exact project — accessible via a QR code on the box, with WhatsApp and email backup. We've spent 10 years building projects for makers, schools, and institutions across India. If a part fails because of a manufacturing defect, replace it free within 7 days.
What if I get stuck during the build?
Scan the QR code on the box to launch the AI companion, or message us on WhatsApp. The companion recognizes your stage from your query and gives specific troubleshooting steps for this enzyme kinetics setup, from wiring checks to calibration curves.
How do I calibrate the turbidity sensor for enzyme reactions?
The included protocol uses a dilution series of a known turbidity standard. The AI companion walks you through recording sensor voltages, plotting a standard curve, and implementing the regression equation in your Arduino code so that readings convert directly to absorbance or NTU.
What enzyme-substrate systems work best with this kit?
Any reaction that changes solution turbidity as product forms or substrate clears. Common examples are amylase-catalyzed starch breakdown, protease digestion of casein, or lipase-mediated clearing of lipid emulsions. The sensor’s 0–3000 NTU range covers typical undergraduate and graduate-level experiments.
How do I obtain Km and Vmax from the SD card data?
After each substrate concentration run, transfer the CSV file to a computer. Use the provided Python script or Excel template to calculate initial reaction velocities (V0) and perform Michaelis-Menten non-linear regression. The companion explains each step, including how to set up substrate dilutions for a full kinetics profile.
Turbidity sensor measures product accumulation rate at different substrate concentrations. Fits Km and Vmax.
What's in this kit
Choose your assembly option:
- Soldering Kit — 25W soldering iron, 60/40 solder wire, flux, and small perfboard for permanent assembly.
- Breadboard Combo — 800-point full-size breadboard with 65-piece jumper wire pack for solderless prototyping.
Other projects you can build
Shipping Information
- Prepaid Orders: ₹75 for orders up to ₹999, FREE shipping above ₹999
- COD Orders: ₹125 shipping + ₹50 COD fee = ₹175 total
- Delivery Timeline: Dispatch in 1-2 days, delivery in 2-7 days depending on location
Returns & Warranty
- 7-Day Return: Manufacturing defects only (approval required)
- Warranty: 7 days from delivery
- Non-Returnable: Batteries, consumables, cut wires, clearance items