Enzyme Temperature Optimum Study Kit with Arduino Uno + LED
Enzyme Temperature Optimum Study Kit with Arduino Uno & OLED — Find Reaction Sweet Spot
Every part needed, pre-tested for compatibility, with an AI build companion trained on this exact project. Shipped from Bengaluru in 3-5 days.
Enzymes are biological catalysts whose activity depends heavily on temperature. This kit lets you precisely measure how an enzyme's reaction rate changes as you vary temperature, using two DS18B20 probes to monitor the enzyme solution and water bath, plus a pH module to track product formation. Whether for a CBSE class 12 investigatory project, a Smart India Hackathon prototype, or a B.Tech biotech lab, you'll gather real data and identify the enzyme's optimum temperature — just like in a professional research setup.
What You'll Build
By the end of this 3–4 hour build, you'll have constructed a dual-zone temperature data logger with OLED display and microSD storage. The station uses one DS18B20 probe in the enzyme solution and a second in the water bath to maintain tight thermal control. The analog pH module continuously tracks product formation, so you can calculate reaction rate at every temperature point. All readings update in real time on the 0.96 inch OLED and save to a CSV file with millisecond timestamps from the DS3231 RTC.
What You'll Learn
- Calibrating an analog pH module and converting its voltage output to pH units using buffer solutions
- Wiring and reading multiple DS18B20 temperature probes over OneWire with Arduino
- Structuring time-stamped sensor data on a microSD card for analysis in Excel or Python
- Plotting reaction rate vs. temperature curves to pinpoint the thermal optimum of an enzyme
Kit Contents
| Component | Quantity |
|---|---|
| Arduino Uno R3 | 1 |
| DS18B20 Probe | 2 |
| Analog pH Module | 1 |
| DS3231 RTC | 1 |
| MicroSD Module | 1 |
| 0.96in OLED | 1 |
| 4.7kΩ Resistors | 5 |
| 10kΩ Resistors | 5 |
| 400-pt Breadboard | 1 |
| M-M Wires | 20 |
| 9V Battery Snap | 1 |
Why Buy This Kit Instead of Sourcing Parts Separately
| Factor | Sourcing Separately | Compoden Kit |
|---|---|---|
| Compatibility checks | You verify every part | Pre-tested as a system |
| Build support | Forums and scattered tutorials | AI companion trained on this exact project |
| Time to first working build | Days of debugging | Hours, with step-by-step guidance |
| Shipping coordination | Multiple sellers, multiple delays | One shipment from Bengaluru in 3-5 days |
Who This Kit Is For
This kit is designed for CBSE Class 11–12 students tackling biology or chemistry investigatory projects, B.Tech ECE/Biotechnology undergrads at IITs, NITs, VIT, or BITS Pilani who need a plug-and-play data logging rig, and ATL Tinkering Lab mentors who want a science fusion project. It's equally relevant for Smart India Hackathon teams building precision monitoring prototypes. If you need to graph real enzyme kinetics data — not just simulate it — this kit delivers.
Built and Backed by Compoden
Every Compoden kit ships with an AI build companion trained on this exact project — accessible via a QR code on the box, with WhatsApp and email backup. We've spent 10 years building projects for makers, schools, and institutions across India. If a part fails because of a manufacturing defect, replace it free within 7 days.
What if I get stuck during the build?
Open the AI companion by scanning the QR code on the box; it has troubleshooting steps for every component. You can also message us on WhatsApp — we reply within 2 hours during working days.
How do I control the temperature of the enzyme solution?
Heat water in a simple beaker on a stove or hot plate, then place the enzyme tube in the water bath. One DS18B20 goes inside the enzyme solution, the other in the water bath, so Arduino can compare and help you manually adjust the heat to hold each test temperature steady.
Can I use any enzyme with this kit?
Yes, but we provide a reference protocol for catalase (from liver or potato) and amylase (from saliva or germinating seeds), because these show clear pH changes during reaction. For other enzymes, ensure the pH module has a measurable output over your reaction's pH range.
How do I analyse the logged data?
The microSD card stores a CSV file with columns for timestamp, pH, and both temperatures. Import this file into Google Sheets or Excel, calculate reaction rate (ΔpH/time) for each temperature, and plot rate vs. temperature. The peak of the graph is the optimum temperature.
DS18B20 + pH track enzyme reaction at different temperatures. Finds optimum temperature.
What's in this kit
Other projects you can build
Shipping Information
- Prepaid Orders: ₹75 for orders up to ₹999, FREE shipping above ₹999
- COD Orders: ₹125 shipping + ₹50 COD fee = ₹175 total
- Delivery Timeline: Dispatch in 1-2 days, delivery in 2-7 days depending on location
Returns & Warranty
- 7-Day Return: Manufacturing defects only (approval required)
- Warranty: 7 days from delivery
- Non-Returnable: Batteries, consumables, cut wires, clearance items